Cardiac fibroblasts aid cardiac function by maintaining mechanical, structural and electrochemical properties of the myocardium via interaction with cardiomyocytes. The involvement in cardiac pathology, such as reparative fibrosis of the infarcted heart, underline the potential of this underrated cell type for cardiovascular research and drug development.
Axiogenesis has developed human induced pluripotent stem (iPS) cell-derived cardiac fibroblasts, named Fibro.Cor.4UTM, with characteristic electrochemical function and morphology essential for 2D and 3D co-culture assays with Cor.4U® or CorV.4UTM cardiomyocytes. FibroCor.4U cardiac fibroblasts are a 100% pure cardiac fibroblast cell type and form a syncytium with cardiomyocytes, actively conducting electric impulses via endogenously expressed ion channels. FibroCor.4U cardiac fibroblasts cause a twofold enhancement in contraction force of Cor.4U human iPSC-derived cardiomyocytes and a threefold enhancement in contraction force of CorV.4U human iPSC-derived ventricular cardiomyocytes in 2D and 3D co-cultures.
Advantages of Fibro.Cor.4U human iPSC – derived cardiomyocytes:
- Primary-like cardiac fibroblast of human origin
- Ready-to-use (just thaw and plate)
- Isogenic with other products of the Cor.4U cardiomyocyte family
- Suitable for co-culture assays with Cor.4U or CorV.4U cardiomyocytes
- Applicable for organotypic 3D culture
Fibro.Cor.4U human iPS cell-derived cardiomyocytes have been validated in the following assays and applications:
- Manual patch clamp
- Cell contraction force